Hello @triciabeel,
You have 2 days to submit your direct deposit payroll before the pay or check so your employee will receive the money, regardless of the pay period. Say, your pay or check date falls on Monday and submitted your direct deposit payroll the same day, it'll be posted on your employee's bank account on Wednesday.
You can read the Direct deposit processing times section of this article to learn more: Create paychecks in online payroll.
I"m also adding a reference here for ideas in case you'll bump into errors in processing your direct deposit payroll: Resolve Direct Deposit Issues.
Reach out to me if you need more help. Always around here to assist you!