If you meant the Location field is missing from your forms despite toggling the feature on, browser-related issues are one of the most common reasons UI elements suddenly vanish in QuickBooks Online. I suggest logging into QuickBooks using a different browser to check if the option is available there.
On the other hand, if you are trying to assign a location to a transaction but don't see it on the dropdown menu, you'll want to ensure it's properly set up and not deactivated so it appears in the list.
Please feel free to add a comment below if you meant something else or have any follow-up questions about location tracking in QuickBooks.