Yes, Laurie. It is possible as long as the owner was set up as an employee and paychecks were issued accordingly.
It’s important to note that Line 1 of Form 941 includes a count of employees who received pay during a pay period that included the dates March 12 (for Q1), June 12 (for Q2), September 12 (for Q3), or December 12 (for Q4).
To confirm, review the paychecks issued during the third month of each quarter and check the corresponding pay periods to ensure compliance. Please see the virtual reference below:



To learn more about the other boxes of the 941 form, please check out this article: How QuickBooks populates the 941.
Let us know if you have questions.