Hello there, ileana1.
The vendor direct deposit limit will refresh every 14 calendar days from the date of submission. This includes weekends. Please note that it is based on the submission date and not on the paycheck date.
Furthermore, your company limit will reset every 6 calendar days including weekends. This starts from the date of your submission.
If you wish to learn more about the direct deposit in QuickBooks Desktop, you can check out this article for the complete details: Direct Deposit service agreement. It describes how the service works and its limitations.
In case you want to stop the debit of a vendor direct deposit, kindly refer to the Need to void the vendor direct deposit you just sent? section of this article for the steps you need to take: Pay bills for vendors through Direct Deposit.
Please let me know if you have other concerns. I'm just around to help.